Non-coding RNAs in Hepatocellular Carcinoma
Principal Investigator: Dr. rer. nat. Sven Diederichs
Although non-protein-coding RNAs (ncRNAs) represent the majority of all transcripts in a human cell, the function of ncRNAs are mostly not understood. Based on the initial findings on ncRNA dysregulation in cancer, we will identify ncRNAs that are aberrantly expressed in hepatocellular carcinoma, uncover their expression and localization profiles and characterize their cellular functions. Lastly, we will analyze their molecular function by unraveling the ncRNA-protein interactomes through RNA affinity purification. This study will elucidate the role of selected ncRNAs in molecular hepatocarcinogenesis.
List of relevant publications
- Winter J*, Jung S*, Keller S, Gregory RI, Diederichs S (2009) Many roads to maturity: microRNA Biogenesis pathways and their regulation. Nat Cell Biol 11(3):228-34. [Review]
- Diederichs S, Jung S, Rothenberg SM, Smolen GA, Mlody BG, Haber DA (2008) Coexpression of Argonaute-2 enhances RNA interference toward perfect match binding sites. Proc Natl Acad Sci USA 105:9284-9.
- Diederichs S, Haber DA. (2007) Dual role for Argonautes in microRNA processing and posttranscriptional regulation of microRNA expression. Cell 131:1097-108.
- Diederichs S, Haber DA. (2006) Sequence variations of microRNAs in human cancer: alterations in predicted secondary structure do not affect processing. Cancer Res 66:6097-104.
- Ji P*, Diederichs S*, Wang W, Böing S, Metzger R, Schneider PM, Tidow N, Brandt B, Buerger H, Bulk E, Thomas M, Berdel WE, Serve H, Müller-Tidow C. (2003) MALAT-1, a novel noncoding RNA, and thymosin ß4 predict metastasis and survival in early-stage non-small cell lung cancer. Oncogene 22(39):8031-41